耦合效应下压力型锚索锚固机理的近似理论分析

为了更加精确地研究压力型锚索的锚固机理,基于锚索预应力与岩土体蠕变的耦合效应推导了锚索预应力方程。在此基础上根据Mindlin问题解,推导了耦合效应作用下锚索砂浆体的压应力和剪应力近似解,并考虑锚索预应力与岩土体蠕变之间的耦合效应进行了案例分析。研究结果表明:锚索预应力和砂浆体受到的应力峰值均按反幂函数随时间逐渐衰减并稳定;承压板的长宽比与压应力峰值之间存在正相关关系,与剪应力峰值之间存在负相关关系;岩土体的泊松比与应力峰值之间存在正相关关系。该成果丰富了压力型锚索锚固机理的理论研究,可为边坡锚固设计提供一定的理论依据。     

Quantitative Elemental Analysis of a Single Cell by Using Inductively Coupled Plasma-Mass Spectrometry in Fast Time-Resolved Analysis Mode

The elemental composition of a single yeast, green alga, or red blood cell (RBC) was precisely determined by using inductively coupled plasma-mass spectrometry (ICP-MS) operating in fast time-resolved analysis (TRA) mode. The technique is known as single-cell (SC)-ICP-MS. Phosphorus, sulfur, magnesium, zinc, and iron were detected in the three types of cell. The elemental composition of yeast and green alga obtained by SC-ICP-MS was consistent with results obtained from conventional ICP-MS measurements following acid digestion of the cells. Slight differences were found in the measured values between SC-ICP-MS and the conventional ICP-MS results for RBC. However, the SC-ICP-MS results for S and Fe in RBC were closer to the estimated values for these elements that were calculated from the level of hemoglobin in RBCs. The data suggest that SC-ICP-MS is suitable for the analysis of various cell types, namely, fungus, plant, and animal cells.     

Effective Characterization of DNA Ligation Kinetics by High-Resolution Melting Analysis

High-resolution melting (HRM) analysis has been improved and applied for the first time to quantitative analysis of enzymatic reactions. By using the relative ratios of peak intensities of substrates and products, the quantitativity of conventional HRM analysis has been improved to allow detailed kinetic analysis. As an example, the ligation of sticky ends through the action of T4 DNA ligase has been kinetically analyzed, with comprehensive data on substrate specificity and other properties having been obtained. For the first time, the kinetic parameters (k_obs and apparent K_m) of sticky-end ligation were obtained for both fully matched and mismatched sticky ends. The effect of ATP concentration on sticky-end ligation was also investigated. The improved HRM method should also be applicable to versatile DNA-transforming enzymes, because the only requirement is that the products have T_m values different enough from the substrates.     

Mouse Models of Sjögren’s Syndrome with Ocular Surface Disease

Sjögren’s syndrome (SS) is a systemic rheumatic disease that predominantly affects salivary and lacrimal glands resulting in oral and ocular dryness, respectively, referred to as sicca symptoms. The clinical presentation of ocular dryness includes keratoconjunctivitis sicca (KCS), resulting from the inflammatory damage to the ocular surface tissues of cornea and conjunctiva. The diagnostic evaluation of KCS is a critical component of the classification criteria used by clinicians worldwide to confirm SS diagnosis. Therapeutic management of SS requires both topical and systemic treatments. Several mouse models of SS have contributed to our current understanding of immunopathologic mechanisms underlying the disease. This information also helps develop novel therapeutic interventions. Although these models address glandular aspects of SS pathology, their impact on ocular surface tissues is addressed only in a few models such as thrombospondin (TSP)-1 deficient, C57BL/6.NOD.Aec1Aec2, NOD.H2^b, NOD.Aire KO, and IL-2Rα (CD25) KO mice. While corneal and/or conjunctival damage is reported in most of these models, the characteristic SS specific autoantibodies are only reported in the TSP-1 deficient mouse model, which is also validated as a preclinical model. This review summarizes valuable insights provided by investigations on the ocular spectrum of the SS pathology in these models.     

[可靠性建模及试验技术]重型机床液压元件油液污染退化量分布剖面可靠性建模

针对重型机床液压系统故障频繁且多与油液中的固态颗粒污染物相关的问题，进行了油液污染趋势变化试验。通过时域分析获得了油样颗粒数的有量纲和量纲一参数，通过Q-Q图和K-S检验分析有量纲参数，污染颗粒数是退化量服从正态分布的退化数据。进行了油液污染与环境相关性分析试验，采用相关系数法分析得到，颗粒数变化量与一定范围内的温度、流量、压力的相关性小；将液压元件分为管路、阀、过滤器三类，用直径5 μm左右的颗粒和直径大于15 μm的颗粒分别研究管路及阀件的堵塞和磨损情况，以过滤器过滤精度大小的颗粒研究过滤器的堵塞情况，设定ISO4406标准20/17级对应的颗粒数为阈值，利用退化量分布建立了液压元件单一故障模式的可靠性模型；利用竞争失效模型将上述模型融合为多故障模式下的可靠性模型。     

基于小波分析的谐波在线监测系统

针对传统谐波在线监测系统存的结果不准确，无法描述谐波动态变化特点，设计了基于小波分析的谐波在线监测系统。首先设计了谐波在线监测系统的硬件模块，然后谐波在线监测系统的软件模块，依据小波变换分析谐波信号特征，得到的谐波变化率，从而谐波在线监测系统的监测结果，最后采用具体实验进行了谐波在线监测系统性能的仿真实验，由实验结果可知，该系统的谐波监测精度高，可以有效检测各种谐波分量，保证电力系统稳定运行。     

拉锻式摩擦塞焊的热力耦合解析模型和试验验证

为快速得到拉锻式摩擦塞焊的焊接工艺参数，对焊接过程进行预测，建立了摩擦塞焊过程的扭矩、轴向力、功率和能量等指标的力学模型和热力耦合解析模型。模型的输入量为焊接塞棒的几何参数、材料属性、焊接转速和进给速度，输出量为焊接过程的扭矩、轴向力、功率和能量等。在自主设计的拉锻式摩擦塞焊设备上进行了焊接试验。通过将试验数据的输入和输出指标与理论模型值进行对比分析，验证了理论解析模型的可行性。     

WC-Ni硬质合金密封圈损伤失效研究

某核主泵采用的WC-Ni硬质合金O形密封圈在运行一段时间后,密封圈端面出现较多的裂纹。为研究密封圈端面损伤失效原因,对密封圈损伤区进行微观形貌分析、白光干涉测试分析、损伤区化学成分分析和表面残余应力测试,讨论WC-Ni硬质合金密封圈表面出现的损伤特征,并对其服役安全性进行评估。结果表明:裂纹源多在密封圈槽堰区和坝区的交界处产生,裂纹多数分布在坝区,坝区损伤程度相比槽堰区较大;裂纹区存在氧化现象,但氧化程度比较轻微,氧化产物主要为W的氧化物;裂纹的产生主要是由密封圈槽堰区和坝区之间较大的应力差导致的,但裂纹体积较小,损伤轻微,短期内不会对整体机械密封装备造成重要影响。     

变截面Z型折叠机翼振动特性的有限元与实验分析

Z型变截面折叠机翼作为一种可变体机翼结构,不同的折叠角对机翼稳定性有着重要的影响,因此研究不同折叠角度下的特性参数对机翼动态稳定性有着重要的意义.本文首先设计加工了Z型变截面可折叠机翼结构的实验模型,通过建立与实验模型相匹配的有限元模型,仿真得到不同折叠角度下机翼的前5阶固有频率和振型,针对不同折叠角度下机翼的固有特性,通过扫频实验得到机翼前5阶固有频率和模态振型,以及横向外激励作用下三段翼的频响曲线,对比分析有限元仿真与实验结果,验证结果的可靠性,这将对机翼结构设计以及特性参数的选取提供参考依据.     

带缓冲区且产品保修的设备预防性维护建模

论文考虑的是一个完美的生产系统,即生产系统所生产的产品均没有质量问题。为提高设备的可靠性,调整市场需求,提出带缓冲区的设备预防性维护优化建模方法。已经出售的产品,顾客在使用过程中可能会出现故障,基于这个情况,在保修期内会予以免费维修。找出生产系统的运行时间(T)和缓冲区库存量(S),以此为决策变量建立期望成本模型。在此基础上,构造求解最优策略的算法。通过数值算例演示了该模型,通过灵敏度分析确定了最优策略和相关参数的关系。